Help Wanted
The Paired-End-Tag library construction in the Polonator protocols is simply the first well defined and kit-supported front-end protocol for the Polonator. We reach out to the world-wide community of researchers and genome centers to develop and publish additional front-end protocols for the Polonator. These might include, but are not limited to:
- basic RNA quantitation (PMAGE)
- allele-specific RNA quantitation
- RNA splicing
- microRNAs
- single cell analyses
- exon enrichment
- genome-wide association studies
- megabase-region selective sequencing
- DNA haplotyping
- DNA methylation; BS-Seq
- Chromatin immuno-precipitation (ChIP-seq)
- Microbiomics
- VDJ-omes
- cancer panels
- rearrangement measures using paired-end-tags (PET)
- aptamer libraries
- microbial genome lab evolution
- population variation in small genomic regions.
We look forward to the genomic community embracing the Polonator as a low cost, high performance sequencing solution, and broadening the protocol base well beyond the current and upcoming efforts of the Church Lab.